— 187 — • 



20.25 e. e. of "'5 NaOH, corresponding to 0.01119 « o phosphorus 

 in filtrate A ; 



13.9 e. e. of "'5 NaOH, corresponding to 0.00768 «o phosphorus in 

 filtrate C. 



Results an increase of 0.00351 grms. phosphorus in 100 e. e. of 

 filtrate A. 



Experi me nt No. 9. 



The brain of an other dog was prepared as already described. The 

 weight of the brain was 50 grms. 



Research on ferments. To this end the foUowing mixtures 

 were made : 



A : 22 grms. of brain paste were mixed with 220 e. e. of chlo- 

 roform water adding besides 2.2 e. e. of pure chloroform. (Main re- 

 search). 



C : 22 grms. of brain paste were mixed with 220 e. e. of water 

 and heated to boiling point. When cool 2.2 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37° for 96 hours. 



On the filtrates A and C, obtained as before, the following deter- 

 minations were made : 



1. 40 e. e. of filtrate A gave 0.1264 grms. i. e. 3.16 ° 00 dry residue; 

 40 e. e. of filtrate C gave 0.0972 grms. i. e. 2.43 0/00 dry residue; 

 The residues incinerated left : 



0.0400 grms. i. e. 1 %o ash in filtrate A; 



0.0376 grms. i. e. 0.94 o'oo ash in filtrate C; 



and consequently : 



0.0864 grms. i. e. 2.16^00 organic matters in filtrate A; 



0.0596 grms. i. e. 1.49» 00 organic matters in filtrate C. 



2. Nitrogen estima ti on. In 20 e. e. of each filtrate the ni- 

 trogen was estimated. 



To neutralise the ammonia formed were required : 



3.95 e. e. of "/,„ HCl, which correspond to 0.0276 % nitrogen in 

 filtrate A ; 



2.8 e. e. of »/,,, HCl, which correspond to 0.0196 «o nitrogen in 

 filtrate C. 



Results an increase of 0.0080 grms. nitrogen in 100 e. e. of fil- 

 trate A. 



3. Furine nitrogen estimation. The ammoniaca! Silver ni- 

 trate yielded in 100 e. e. of filtrate A a precipitate which required, ac- 



