' — 188 — 



cording to Kjeldahl, 1.65 e. e. of "/lO HCl, corresponding to 0.00231 o/o 

 piirine nitrogen. 



No precipitate of Silver compound of the purin-bases was obtai- 

 ned in control filtrate C. 



4. Phosphorus estimation. To dissolve the molybdenum pho- 

 sphate resulting from 40 e. e. of each filtrate were required : 



18.8 e. e. of "'5 NaOH, which correspond to 0.01039 per cent pho- 

 sphorus in filtrate A ; 



13.75 e. e. of "/j NaOH, which correspond to 0.00760 per cent pho- 

 sphorus in filtrate C. 



Results an increase of 0.00279 grms. phosphorus in 100 e. e. of 

 filtrate A. 



Experiment No. 10. 



An other brain was cleaned and reduced to a homogeneous paste 

 as before. 



It weighed 49 grams. 



Research on ferments. Por this purpose the following two 

 mixtures were made : 



A. 22 grins. of brain paste were mixed with 220 e. e. of chlo- 

 roform water adding besides 2.2 e. e. of pure chloroform (Main-research). 



C, 22 grms. of brain paste were mixed with 220 ce. of water 

 and heated to boiling point. After cooling 2.2 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37" for 96 hours. 



On the filtrates A and C, obtained as before, the following deter- 

 minations were made : 



1. 40 e. e. of filtrate A gave 0.1264 grms. of dry residue i. e. 

 3.16 « 00; 



40 e. e. of filtrate C gave 0.0912 grms. of dry residue , i. e. 

 2.28 o'oo. 



These residues incinerated left : 



0.0412 grms. of ash, i. e. 1.03 " 00, in filtrate A; 



0.0380 grms. of ash, i. e. 0.95 «/oo, in filtrate C; 



and consequently: 



0.0852 grms. of organic matters, i. e. 2.13 ^loo, in filtrate A; 



0.0532 grms. of organic matters, i. e. 1.33 0/00, in filtrate C. 



2. Nitrogen estimation. In 20 e. e. of each filtrate the ni- 

 trogen was estimated. 



To neutralise the ammonia resulting were required: 



