— 189 — 



3.3 e. e. of "',„ HCl, which correspond to 0.0231 "o nitrogen in 

 filtrate A ; 



2.15 e. e. of "jo HCl, which correspond to 0.0150 "'o nitrogen in 

 filtrate C. 



Results an increase of 0.0081 grms. nitrogen in 100 e. e. of fil- 

 trate A. 



3. Furine nitrogen estimation. The precipitate of Silver 

 compound, obtained in 100 e. e. of filtrate A, required, according to 

 KjELDAHL, 2.15 C.C. of ", jo HCl, w'hich correspond to 0.00301 grms. pu- 

 rine nitrogen. 



No purin-bases were precipitated in filtrate C. 



4. Phosphorus estimation. The phosphorus was estimated 

 in 40 e. e. of each filtrate. 



To dissolve the moiybdenum phosphate precipitated were required: 



22.05 e. e. of "'=, NaOH, corresponding to 0.01219 per cent pho- 

 sphorus in filtrate A ; 



15.20 e. e. of "/s NaOH, corresponding to 0.00840 per cent pho- 

 sphorus in filtrate C. 



Therefore results an increase of 0.00379 grms. phosphorus in 100 

 e. e. of filtrate A. 



Experi me nt No. 11. 



A brain weighing 57 grms. prepared as before was employed for 

 this experiment. 



Research on ferments. With the paste obtained the following 

 two mixtures were made : 



A : 25 grms. of brain paste were mixed with 250 e. e. of chloro- 

 form water adding besides 2.5 e, e. of pure chloroform (Main-research). 



C : 25 grms. of brain paste were mixed with 250 e. e. of water 

 and heated to boiling point. When cool 2.5 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37" for, 96 hours. 



The filtrates A and C were obtained as in previous experiments. 



1. 40 e. e. of filtrate A gave 0.1260 grms. of dry residue i. e. 



3.15 o'oo; 



40 e. e. of filtrate C gave 0.0904 grms. of dry residue i. e] 



2.26 o/oo. 



The residues incinerated left : 



0.0432 grms. of ash. i. e. 1.08 "oo, in filtrate A; 



0.0404 grms. of ash, i. e. 1.01 "'oo, in filtrate C; 



