— 190 — 



and consequently : 



0.0828 grms. of organic matters, i. e. 2.07 o/oo, in filtrate A ; 



0.0500 grms. of organic matters, i. e. 1.25 o'oo, in filtrate C. 



2. Nitrogen estimation.To neutralise the ammonia resul- 

 ting from 20 e. e. of each filtrate were required : 



3.4 e. e. of "/io HCl, corresponding to 0.0238 o/q nitrogen in fil- 

 trate A; 



2.15 e. e. of "io HO, corresponding to 0.0150% nitrogen in fil- 

 trate C. 



Results an increase of 0.0088 grms. nitrogen in 100 e. e. of fil- 

 trate A. 



3. Furine nitrogen estimation. The purine nitrogen was 

 estimated in 100 e. e. of filtrate A. The Silver compound formed re- 

 quired, according to Kjeldahl, 1.7 e. e. of "io HCl, which corre- 

 spond to 0.00238 Oq purine nitrogen. 



No precipitate was yielded by the ammoniacal Silver nitrate in fil- 

 trate C. 



4. Phosphorus estimation. To dissolve the molybdenum pho- 

 sphate, resulting from 40 e. e. of each filtrate, required : 



20.8 e. e. of "/j NaOH, which correspond to 0.01150 per cent pho- 

 sphorus in filtrate A ; 



14.45 e. e. of "/s NaOH, which correspond to 0.00799 per cent pho- 

 sphorus in filtrate C. 



Results an increase of 0.00351 grms. phosphorus in 100 e. e. of 

 filtrate A. 



Experiment No. 12. 



An other brain w^ar prepared as already described. It weighed 

 58 grms. 



Research on ferments. To this end, two mixtures were made 

 as follo WS : 



A: 25 grms.^of brain paste were mixed with 250 e. e. of chloro- 

 form water adding besides 2.5 e, e. of pure chloroform (Main-research). 



C : 25 grms. of brain paste were mixed with 250 e. e. of water 

 and heated to boiling point. When cool 2.5 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37" for 96 hours. 



On the filtrates A and C, obtained as before mentioned, the follo- 

 wing determinations were made : 



1 . 40 e. e. of filtrate A gave 0.1356 grms. of dry residue, i. e. 3.39 «/oo; 



