— 191 — 



40 e. e. of filtrate C gave 0.0984 grms. of dry residue, i. e. 2.46 °oo; 



The residues incinerated left : 



0.0448 grms. of ash, i. e. 1.12 "o,,, in filtrate A; 



0.0420 grms. of ash, i. e. 1.05 " ,„. in filtrate C; 



and consequently : 



0.0908 grms. of organic matters, i. e. 2.27 "oo in filtrate A ; 



0.0564 grms. of organic matters, i. e. 1.41 o/oo in filtrate C. 



2. Nitrogen estima ti on. The nitrogen was estimated in 20 

 e. e. of each filtrate. 



To ncutralise the ammonia resulting were required : 



3.9 e. e. of ", ,0 HCl, which correspond to 0.0273 "',> nitrogen in 

 filtrate A ; 



2.5 e. e. of ",',0 HCI, which correspond to 0.0175% nitrogen in 

 filtrate C. 



Results an increase of 0.0098 grms. nitrogen in )00 e. e. of fil- 

 trate A. 



3. Furine nitrogen estimation. The precipitate of Silver 

 compound formed in 100 e. e. of filtrate A required, according to Kjel- 

 DAHL, 1.8 C.C. of "/io HCl, which correspond to 0.00252 Vo purine ni- 

 trogen. 



No purine bases have been precipitated by the ammoniacal Silver 

 nitrate in the filtrate of the control-research. . 



4. Phosphorus estimation. The phosphorus was estimated in 

 40 e. e. of each filtrate. To dissolve the precipitate of molybdenum 

 phosphate resulting were required : 



21.5 e. e. of n/5 NaOH, which correspond to 0.01188 «o phospho- 

 rus in filtrate A ; 



14.65 e. e. of "'5 NaOH, which correspond to 0.00810 o'o phospho- 

 rus in filtrate C. 



Results an increase of 0.00378 grms. phosphorus in 100 e. e. of 

 filtrate A. 



Experiment No. 13. 



An other brain was prepared as in experiment No. 1. It weighed 

 62 grms. 



Research on ferments. Por this purpose the following t\xo 

 mixtures were made : 



A : 28 grms. of brain paste were niixed with 280 e. e. of chloro- 

 form water adding besides 2.8 e. e. of pure chloroform (Main-research). 



C : 28 grms. of brain paste were mixed with 280 e. e. of water 



