— 192 — 



and beateci to boiling point. VVhen cool 2.8 e. e. of pure chloroform 

 were added (control-research). 



Both mixtures Avere left to incubate at 37° for 96 hours. 



On the filtrates A and C, obtained as in previous experiments, the 

 following determinations were made: 



1. 40 e. e. of filtrate A gave 0.1276 grms. of d'ry residue, i. e. 3.19 o/oq, 

 40 e. e of filtrate C gave 0.0980 grms. of dry residue, i. e. 2.45 o/oo- 



which incinerated left : 



0.0436 grms. of ash, i. e. 1.09 o/oo in filtrate A; 



0.0404 grms. of ash, i. e. 1.01 o/oo in filtrate C; 

 and consequently : 



0.0840 grms. of organic matters, i. e. 2.1o/oo in filtrate A; 



0.0576 grms. of organic matters, i. e. 1.44 o/qq in filtrate C. 



2. Nitrogen estimation. The nitrogen was estimated in 20 

 e. e. of each filtrate. To neutralise the ammonia resulting were required: 



3.7 e. e. of "/lo HCl, corresponding to 0.0259 o/o nitrogen in fil- 

 trate A; 



2.55 e. e. of "/lo HCl, corresponding to 0.0178 "/o nitrogen in fil- 

 trate C. 



Results an increase of 0.0U81 grms. nitrogen in 100 e. e. of fil- 

 trate A. 



3. Furine nitrogen estimation. The Silver compound of 

 the purin-bases precipitated in 100 e. e. of filtrate A required, accor- 

 ding to KjELDAHL, 2 e. e. of "/io HCl, which correspond to 0.0028 o/o 

 purine nitrogen. 



No precipitate of Silver compound was obtained in the filtrate of 

 the control-research. 



4. Phosphorus estimation. The phosphorus was estimated in 

 40 e. e. of each filtrate. To dissolve the precipitate of molybdenum 

 phosphate resulting were required : 



21.1 e. e. of "/^ NaOH, which correspond to 0.01 166 o/o phospho- 

 rus in filtrate A; 



14.1 e. e. of "'5 NaOH, which correspond to 0.00779% phosphorus 

 in filtrate C. 



Results an increase of 0.00387 grms. phosphorus in 100 e. e. of 

 filtrate A. 



