— 176 — 



water and then with water. The filter with the precipitate was 

 dried to 120° and then kjeldahhzed. 



6. The phosphor u s was estimated according toNEUiMANN. 

 The precipitate of molybdenum phosphate was dissolved in a "/r, 

 sodium hydrate solution and the excess of alkah was titrated 

 with a "/io hydrochloric acid solution employing the phenol- 

 phtalein as indicator. 



III. — Description of the Experiments, 

 E X p e r i m e n t No. 1 . 



The brain of a dog fieshly killed was freed from its adhering mem- 

 branes and blood, then triturated in a mortar to a homogeneous paste. 

 It weighed 52 grms. 



The foUowing determinations were made on the brain paste : 



a. 5 grms. of brain paste gave 0.9746 grms. of dry residue i. e. 

 19.492 o/o, wich incinerated left 0.0693 grms. of ash i. e. 1.386 «'o; and 

 consequently result 0.9053 grms. of organic matters i. e. 18.106 °o- 



b. Total nitrogen estimation. 1. 5946 grms. of brain paste 

 required, according to Kjeldahl, 17.55 e. e. of "/^o HCl, corresponding 

 to 1.54 °/o nitrogen, and 



1.9512 grms. of brain paste required 21.65 e. e. of " jy HCl cor- 

 responding to 1.55 °o nitrogen. 



Hence, in average, this brain contains 1.545 °o nitrogen. 



e. Phosphor US estimation. 1. 1988 grms. of brain paste re- 

 quired, according to Neumann, 21.9 e. e. of " '^ NaOH , which corre- 

 spond to 0.404 «/o phosphorus and 



0.9178 grms. of brain paste required 16.2 ce. of "/j NaOH, which 

 correspond to 0.390 °/o phosphorus. 



Hence, in average, this brain contains 0.397 «/o phosphorus. 



d. Research on ferments.To this end the foUowing two 

 mixtures were made : 



A : 22 grms. of brain paste were mixed with 220 e. e. of chloro- 

 form water adding besides 2.2 e. e. of pure chloroform (Main-research). 



C : 22 grms. of brain paste were mixed with 220 e. e. of \xater 

 and heated to boiling point. After cooling 2. 2 e. e. of pure chloro- 

 form were added (Control-research). 



► Both mixtures were put together in the incubator at 37° and left 

 there for 96 hours, shaking them now and then. The period of incu- 

 bation over, mixture A was heated to boiling point. When cool both 



