— 177 — 



mixtures were filled with water to 220 e. e. , well shakeri and then 

 filtered through a dry filler. In the filtrates A and C, thus obtained, the 

 following determinations were made : 



1. 40 e. e. of filtrate A gave 0.1312 grms. of dry residue i. e. 

 3.28 o/oo ; 



40 e. e. of filtrate C gave 0.0992 grms. of dry residue i. e. 2.48 » oo- 



The residues incinerated left : 



0.0440 grms. of ash i. e. 1.1 o oo in filtrate A; 



0.0420 grms. of ash i. e. 1.05 o'oo in filtrate C; 



and consequently : 



0.0872 grms. of organic matters i. e. 2.18 o'oo in filtrate A; 



0.0572 grms. of organic matters i. e. 1.43 °'oo in filtrate C, 



2. Nitrogen estimation. The nitrogen was estimated in 20 

 e. e. of each filtrate. To neutralise the ammonia resulting were required: 



3.9 e. e. of " lu HCl, corresponding to 0.0273 o/o nitrogen in fil- 

 trate A; 



2.6 e. e. of " io HCl, corresponding to 0.0182 o;, nitrogen in fil- 

 trate C. 



Results an increase of 0.0091 grms. soluble nitrogen in 100 e. e. 

 of filtrate A, coresponding to 10' grms. of brain. 



3. Furine nitrogen estimation. The precipitate of Silver 

 compound of the purin bases, formed in 100 ce. of filtrate A, was kjel- 

 dahlized. To neutralise the ammonia resulting were required 1.85 e. e. 

 of " jo HCl, which correspond to 0.00259 °/o parine nitrogen in filtrate A. 



No precipitate of purin bases was yielded, in filtrate C, by the am- 

 moniacal Silver nitrate. 



4. Phosphorus estimation. To dissolve the precipitate of mo- 

 lybdenum phosphate formed in 40 e. e. of each filtrate , according to 

 Neumann, were required : 



21.9 e, e. of " /j NaOH, corresponding to 0.01211 per cent pho- 

 sphorus in filtrate A ; 



15.4 e. e. of "5 NaOH, corresponding to 0.00851 per cent pho- 

 sphorus in filtrate C. 



Results an increase of 0.00360 grms. phosphorus in 100 e. e. of fil- 

 trate A, corresponding to 10 grms. of brain. 



Experiment No. 2. 



The brain of an other dog was prepared as already mentioned. It 

 weighed 53 grms. On the brain paste obtained were made the follo- 

 wing determinations : 



