— 178 — 



a. 5 grms. of brain paste gave 0.8722 grms. of dry residue i. e. 

 17.44°,,. The dry residue incinerated left 0.0676 grms. ash i. e. 1.352 o/o; 

 and consequently : 0.8046 grms. organic matters i. e. 16.092 "/o- 



b. Total nitrogen estimation. 1.3258 grms. of brain paste 

 required, according to Kjeldahl, 14.35 e. e. of " 'i„ HCl, which corre- 

 spond to 1.44 o/o nitrogen, and 



1.7702 grms. of brain paste required 18.95 e. e. of "/io HCl which 

 correspond to 1.49 o/o nitrogen. 



Hence, in average, the brain contains 1.46 o/o nitrogen. 



e. Phosphorus estimation. 0.9214 grms. of brain paste re- 

 quired, according to Neumann, 13 e. e. of "/j NaOH corresponding 

 to 0.312 o o phosphorus, and 



1.5716 grms. of brain paste required 20.9 e. e. of "/■; NaOH cor- 

 responding to 0.294 o/o phosphorus. 



Hence, in average, this brain contains 0.303 o o phosphorus. 



d. Research on ferments. For this purpose the following 

 two mixtures were made : 



A : 22 grms. of brain paste were mixed with 220 e. e. of chloro- 

 form water adding besides 2.2 e. e. of pure chloroform (Main-research). 



C : 22 grms. of brain paste were mixed with 220 e. e. of water 

 and heated to boiling point. When cool 2. 2 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37° for 96 hours. The pe- 

 riod of incubation over, mixture A was heated to boiling point and, 

 continuing in the way already mentioned, the filtrates A and C were 

 obtained. The following determinations were made : 



1. 40 e. e. of filtrate A gave 0.1248 grms. of dry residue i e. 

 3. 12 0;oo; 



40 e. e. of filtrate C gave 0.0908 grms. of dry residue i. e. 2.27 o oo. 



The residues incinerated left : 



0.0428 grms. of ash i. e. 1.07 o/qo in filtrate A; 



0.0396 grms. of ash i. e. 0.99 o/oo in filtrate C; 



and consequently : 



0.0820 grms. of organic matters i. e. 2.05 o/oo in filtrate A ; 



0.0512 grms. of organic matters i. e. 1.28 o/oo in filtrate C. 



2. Nitrogen estimation. 20 e. e. of each filtrate were kjel- 

 dalized. To neutralise the ammonia resulting were required : 



3.8 e. e. of "/lu HCl, which correspond to 0.0266 o/o nitrogen in 

 filtrate A; 



2.55 e. e. of "/,„ HCl, which correspond to 0.0178 o/o nitrogen in 

 filtrate C. 



