— 179 — 



Results an increase of 0.0088 gnns. nitrogen in 100 e. e. of filtrate 

 A, corresponding to 10 grms. of brain. 



3. Furine nitrogen estimation. The silver compound, pre- 

 cipitated in 100 e. e. of filtrate A, required according to Kjkldahl, 1.7 

 e. e. of " ly HCl, which correspond to 0.00238° o purine nitrogen. 



The ammoniaca! silver nitrate yielded no precipitate in the filtrate 

 of the control-research. 



4. Phosphorus estimation. The phosphorus was estimated 

 in 40 e. e. of each filtrate, according to Neumann. To dissolve the pre- 

 cipitate of molybdenum phosphate formed were required : 



18.6 e. e. of "5 NaOH, which correspond to 0.01028 o/o phospho- 

 rus in filtrate A ; 



12.5 e. e. of " 5 NaOH, which correspond to 0.00691 ° o phospho- 

 rus in filtrate C. 



Results an increase of 0.00337 grms. phosphorus in 100 e. e. of 

 filtrate A, corresponding to 10 grms. of brain. 



E X p e r i m e n t No. 3. 



An other brain was prepared and treated as in the previous expe- 

 riments. It weighed 52 grms. The following determinations were made 

 on the brain paste obtained : 



a. 5 grms. of brain paste gave 0.9517 grms. of dry residue, i. e. 

 19.034 °o, which incineratcd left 0.0704 grms. of ash, i. e. 1.408 °o; 

 and consequently 0.8813 grms. of organic matters, i. e. 17.626 °/o. 



b. Total nitrogen estimation. 1.1044 grms. of brain paste 

 required, according to Kjeldahl, 12.55 e. e. of "/i„ HCl, corresponding 

 to 1. 59°'o nitrogen, and 



1.5500 grms. of brain paste required 17.7 e. e. of "'(„ HCl, corre- 

 sponding to 1.59 °;'o nitrogen. 



Therefore, the brain contains 1.59 °o total nitrogen. 



e. Phosphorus estimation. 0.9796 grms. of brain paste re- 

 quired, according to Neumann, 12.6 e. e. of "5 NaOH, corresponding 

 to 0.284 "o phosphorus, and 



1,3056 grms. of brain paste required 16 e. e. of "-., NaOH, cor- 

 responding to 0.271 ° o phosphorus. 



Therefore, in average, the brain contains 0.278 «/o phosphorus. 



d. Research on ferments. Por this purpose two mixtures 

 were made as folloxxs : 



A : 22 grms. of brain paste were mixed with 220 ce. of chloroform 

 water, adding besides 2.2 e, e. of pure chloroform (Main-research). 



