— 180 — 



C : 22 grms. of brain paste were mixed wtth 220 e. e. of water 

 and heated to boiling point. After cooling 2. 2 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37° for 96 hours. 



On the filtrates A and C, obtained in the same way as in previous 

 experiments, the following determinations were made : 



1. 40 e. e. of filtrate A gave 0.1364 grms. of dry residue, i. e. 

 3.41 o/oo ; 



40 e. e. of filtrate C gave 0.1000 grms. of dry residue i. e. 2.50o qq. 



The dry residues incinerated left : 



0.0408 grms. ash i. e. 1.02 o/oo in filtrate A; 



0.0392 grms. ash i. e. 0.98 o'oo in filtrate C ; 



and consequently : 



0.0956 grms. of organic matters i. e. 2.39 °/oo in filtrate A; 



0.0608 grms. of organic matters i. e. 1.52 o/qo in filtrate C. 



2. Nitrogen estimation. 20 e. e. of each filtrate were kjel- 

 dahlized. To neutralise the ammonia resulting were required : 



4 e. e. of "/io HCl, wich correspond to 0.0280% nitrogen, in fil- 

 trate A; 



2.7 e. e. of "/i,, HCl, which correspond to 0.0189 % nitrogen, 

 in filtrate C. 



Results an increase of 0.0091 grms. nitrogen in 100 ce. of filtrate A. 



3. Furine nitrogen estimation. The Silver compound ob- 

 tained in 100 e. e. of filtrate A required, according to Kjeldahl, 1.8 e. e. 

 of "/io HCl, corresponding to 0.00252 % purine nitrogen. 



No Silver compound of the purin-bases was precipitated in fil- 

 trate C. 



4. Phosphorus estimation. The phosphorus was estimated in 

 40 e. e. of each filtrate according to Neumann. To dissolve the molyb- 

 denum phosphate precipitated were required : 



'^0.25 e. e. of "/-, NaOH, which correspond to 0.011 19 °'o phospho- 

 rus in filtrate A ; 



13.55 e. e. of n/. NaOH, which correspond to 0.00749 °'o phospho- 

 rus in filtrate C. 



Results an increase of 0.00370 grms. phosphorus in filtrate A. 



Experiment No. 4. 



The brain of an other dog , freshly killed , was pounded in a 

 mortar to a homogeneous paste. It weighed 66 grams. The following re- 

 searches were made on the paste : 



