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a. 5 grms. of brain paste gave 1.0866 grms. of dry residue, i. e. 

 21.730/0; The dry residue incinerated left 0.0742 grms of ash, i. e. 

 1.484 "'0 ; and consequently 1.0124 grms. of organic matters, i. e. 20.25 °'o 



b. Nitrogen estimation. 1.8466 grms. of brain paste requi- 

 red, according to Kjeldahl, 21.3 e. e. of ",'10 HCl, which correspond 

 to 1.61 °;o total nitrogen in the brain. 



e. Phosphorus estimation. 1.7676 grms. of brain paste re- 

 quired, according to Neumann, 31.5 e. e. of "/,- NaOH which corre- 

 spond to 0.394 o'o phosphorus in the brain. 



d. Research on ferments. The following two mixtureswere 

 made : 



A. 25 grms. of brain paste were mixed with 250 e. e. of chloro- 

 form water adding besides 2.5 e. e. of pure chloroform (Main - research). 



C. 25 grms. of brain paste were mixed with 250 e. e. of water 

 and heated to boiiing point. When cool 2.5 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37» for Q6 hours. 



On the filtrates A and C, obtained as already described, the follo- 

 wing determinations were made : 



1. 40 e. e. of filtrate A gave 0.1268 grms. of dry residue, i. e. 

 3.170/00; 



40 e. e. of filtrate C gave 0.0964 grms. of dry residue, i. e. 

 2.41 0/00. 



The dry residues incinerated left : 



0.0448 grms. of ash i. e. 1.12 0/00 in filtrate A; 



0.0412 grms. of ash i. e. 1.03 «/co in filtrate C; 



and consequently : 



0.0820 grms. of organic matters, i. e. 2.05 o/oo in filtrate A; 



0.0552 grms. of organic matters, i. e, 1.38 0/00 in filtrate C. 



2. Nitrogen estimation. The nitrogen was estimated in 20 

 e. e. of each filtrate. To neutralise the ammonia resulting were required: 



3.8 e. e. of " ',,, HCl, which correspond to 0.0266 "o nitrogen, in 

 filtrate A; 



2.7 e. e. of"',„ HCl, which correspond to 0.0189 o/o nitrogen, in 

 filtrate C. 



Results an increase of 0.0077 grms. nitrogen in 100 e. e. of filtrate A. 



3. Furine nitrogen estimation. The Silver compound, ob- 

 tained in 100 e. e. of filtrate A, required, according to Kjeldahl, 1.7 

 e. e. of "/i„ HCl, corresponding to 0.00238 o/o purine nitrogen. 



No purin bases were precipitated in filtrate C. 



4. Phosphorus estimation. The phosphorus was estimated in 



