/l/ The ranges of the concentrations of compounds as given in column B Include the quantities specified for 

 the media of Knop, Benecke, Molisch, Mlquel, and Beijerinck. These have been widely used for cultivation of re- 

 latively hardy green and other algae. Differences between these media are probably not significant for most 

 purposes. Ca(N05)2 or NHljNOj may be substituted for KNO3 (a few algae require ammonia nitrogen); KHgPOl; may be 

 substituted for K2BP0i| for those algae which prefer an acidic environment; CaSOi( may be used Instead of CaCl2, 

 and FeSOl^. may replace FeCl2. Addition of 0.1^ glucose and 0.2^ beef extract is useful for maintenance of stock 

 cultures. For marine algae natural sea water or artificial sea water (cf . column H) may be used with the nedlum. 

 /2/ Medium Is excellent for the widely-grown Chlorella. pH adjusted to l*.5-6.8. MgS0i^.7H20 may be reduced to 50 

 to prevent precipitation on autoclaving. Carbon is best provided by passing 5^ CO2 In air through the cuJ.ture. 

 /?/ Similar media, e.g., Warburg's and Emerson's are available in the literature (ref. 10,11). /I*/ Used in the 

 cultivation of the nitrogen- fixing algae. Drewes' medium which is similar is available in the literature 

 (ref. 2). /5/ Used for the cultivation of planktonic algae (blue-green, greens, diatoms). Similar media, e.g., 

 Richter's, Pringshelm's, and Harden's are available in the literature (ref. 2). /6/ The addition of 100 mg/lOO 

 ml sodium glutamate is desirable for some algae. /7/ The formula Includes various artificial sea water media, 

 e. g., Perrler's, Herbst's, and Pringshelm's. /8/ Not specified as part of named solution, but added as a com- 

 ponent of Arnon's micronutrlent solution B7, for beneficial effect on growth. /9/ Not specified as part of 

 named solution, but added as a component of Arnon's micronutrlent solution Al*, for possible beneficial effect 

 on growth. /lO/ Also: 100- 5OO mg/lOO ml, CaCOj. 



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