/l/ A discuBdlon of the meaning of "germ- free", and a description of the methods used In raising 

 germ- free anlmalB will be found In the Lobund reports of the University of Notre Dame. /2/ It 

 should be noted that each of these columns (B and C) contains data for both control and gena-free 

 chickens. No difference was found between the daily nutrient allowance of controls and geno-free 

 chickens in the case of the two breeds, bantam and white leghorn. For a diet that will supply the 

 nutrients listed in these colunns, see table 50, Diets; Laboratory and Domestic Animals, /j/ Nut- 

 rient allowances for growth, maintenance, reproduction. /U/ Suckling rats. /?/ The values in 

 this column are based on the chemical coii^>osltion of normal rat milk. /6/ Artificially fed. Ill 

 Kllocalorles, calculated from the following standards: protein, h.Z calories per gram; carbohy- 

 drate, U.I5 calories per gram; and fat, 9.'* calories per gram. /8/ One I. U. • 0.6 jig p-carotene, 

 /9/ Vitamin Bi2' /lO/ Calculated as D5. One International Chick Unit = 1.35 A.O.A.C. units = 

 0.025 Jig vitamin D3 (7-dehydrocholeaterol). /ll/ Calculated aa calciferol. One I. U. = 0.025 Hg 

 calciferol, /12/ As ot-tocopherol. /ij/ Folic acid; pteroylglutamlc acid. /lU/ As menadione, 

 2-methyl-l, ^-naphthoquinone . /l5/ As calcium pantothenate. 



68 



