84 



A. A. KANDUTSCH AND JACK H. STIMPFLING 



required for each of the various iniinunological effects which 

 transplantation antigens are able to cause. Since, as appears 

 certain, transplantation isoantigens are components of the lipid- 

 protein (and possibly also carbohydrate) complex which forms 

 cell membranes, it is likely that the size and chemical complexity 

 of an isolated antigenic molecule may be related to the methods 



Fig. 7. The inhibition of haemagglutina- 

 tion by increasing quantities of venom- 

 treated TSL. Red blood cells used in the 

 assay were collected from A/Sn mice. 

 Cells for negative control tests were from 

 strain B10.D2. 



C57BL/10 anti-Bio.D2 A ▲ 



(C57BL/6X DBA/2)Fi anti-CsH 



used to dissociate the membranes into component parts. TSL, 

 isolated by the relatively mild methods used, may thus contain 

 a relatively simple antigenic segment within a larger, more 

 complex, molecule. The conversion, by snake venom, of TSL 

 into a water-soluble substance which yet retains its capacity to 

 inhibit certain specific haemagglutinating reagents indicates that 

 the intact TSL molecule may not be required for production of 

 this effect. On the other hand, it has been suggested that subsi- 



