82 



A. A. KANDUTSCH AND JACK H. STIMPFLING 



Fig. 5. Effects of some enzymes on the turbidity of 

 a TSL suspension. In experiment A, TSL was sus- 

 pended in 0- iM-tris-maleate buffer pH 7-3. In 

 experiment B, the buffer was o-iM-tris, pH 7-9, 

 except that o- iM-phosphate, pH 6-6, was used 

 when digestion was with pepsin. The concen- 

 tration of TSL was approximately 0-25 mg./ml. 

 Concentrations of enzymes were : trypsin (crystal- 

 hzed), o-i mg./ml.; a-chymotrypsin (from 

 ethanol), o-i mg./ml.; pepsin (crystallized), 0-2 

 mg./ml.; Hpase from porcine pancreas (Cal- 

 Biochem., B grade), 0-2 unit/ml.; and lyophilized 

 snake {Crotalus adamanteus) venom from Ross Allen 

 Reptile Institute, o-o8 mg./ml. 



the presence of a major component and a small amount of a 

 second component (Fig. 6). In phosphate buffer pH 6*3, only one 

 component (mobility 4 -06 x io~^ cm.^ sec. "^ volt~^) was evident. 

 The mobilities of both components at pH 8 • 6 were considerably 



