8o 



A. A. KANDUTSCH AND JACK H. STIMPFLING 



due, at least in part, to the partially auto-oxidized lipid moiety 

 (see Gurd, i960, for a summary of effects of auto-oxidation on 

 absorption spectra of lipoproteins). Auto-oxidation of the lipid 

 moiety is also the most likely explanation for the brown colour 



1.2 



1.0 



.2 



220 



240 



260 280 300 320 



Wavelength (Mu) 



Fig. 4. Ultraviolet absorption spectrum of TSL. TSL was 

 dissolved in o- iN-NaOH at a concentration of 0-20 mg./ml. 



of the lipoprotein which has been observed to increase in intensity 

 with time and handling. 



Modification of TSL by snake venom 



Although TSL appears to be an electrophoretically homo- 

 geneous substance, it seems possible that the entire, intact mole- 

 cule may not be required for antigenicity. In view of this, 

 and because specific modifications may be expected to throw 

 further light upon the structure, and the nature of the antigenic 



