78 A. A. KANDUTSCH AND JACK H. STIMPFLING 



each of the buffer systems and the difference in the concentration 

 of Triton between the sample and the buffer appears in the patterns 

 as a sharp spike following the lipoprotein peak. This boundary 

 migrates at a rate not markedly different from that of the lipo- 

 protein. The TSL migrated as a single component in the two 



ASCENDING 



DESCENDING 



— > 65min. — > 110 min. 110 min. < 



Barbital Buffer, pH 8.6: Mobility = -3.24 x I0"5 Cm 2 SeCVolf 



111 



140 rnin . 



190 min. 



190 min. 



Phosphate Buffer, pH 6.3:Mobili1y =-1.94 x lO'S Cm2 Sec"' Volt"' 



Fig. 3. Tracings of electrophoretic patterns of TSL. Freeze- 

 dried TSL was dissolved in buffer solution containing i per cent 

 Triton and dialysed overnight versus the same buffer solution. 

 The concentration of TSL was i • 3 per cent and i per cent in 

 barbitone and phosphate buffers, respectively. Field strength 

 was 13 volts/cm. 



buffer systems shown and also in tris buffer of ionic strength o* i, 

 pH 7-5. An attempt to examine its electrophoretic characteristics 

 at pH 5 in acetate buffer was unsuccessful since the lipoprotein 

 was precipitated from solution under these conditions. Estimations 

 of the isoelectric point from the hmited mobihty data obtained so 

 far suggest that it falls within the pH range 4 and 4*2, a range 



