Fig. 2. Agar ditfusion line patterns of ascitic fluid, H-2 antigen, and intermediate fractions 



tested against 



(a) rabbit antiserum against normal mouse serum (in central wells) ; 



(b) rabbit antiserum against mouse cells, after absorption with normal mouse serum 



(in central wells). 



Peripheral wells all contain 330 [xg. of antigen, labelled thus : 



A = Ascitic fluid. 

 B = Dialysis precipitate. 

 C = Spinco precipitate. 

 D=TM2 gradient fraction. 

 E = I volume water dilution precipitate. 

 F = Dialysis supernatant. 

 G = Spinco supernatant. 

 H= Pooled top plus bottom gradient 

 fractions. 



To face p. 58 



