STUDIES ON TRANSPLANTATION ANTIGENS II 



problem, but two. For the isolation and purification of an active 

 ingredient, much would be achieved if the preparation could be 

 made to pass through a soluble stage, even if the matter finally 

 used in biological tests were to be insoluble. It is therefore a 



Table II 



Composition of antigenic sediment 

 (All figures represent percentages) 



Amino 



(i) Anthrone reaction (Paul, 1958) after correction for RNA ribose, using galactose 

 standard. Sugars identified: galactose, mannose, ribose; traces of glucose and fucose. 

 Mannose: galactose ratio about i :2. 



(2) Neuhaus and Letzring's modification (1957) of Elson-Morgan reaction; after hydrolysis 

 with 2N-HC1 for 3 hours. Amino sugars identified: glucosamine and galactosamine 

 (approximately 1:1). 



(3) Kjeldahl: Paul's (1958) procedure, without fractionation. 



(4) Total protein = nitrogen x 6-25. 



(5) Fraction soluble in lipid solvents using Paul's (1958) fractionation procedure; Bloor's 

 chromic acid method using recrystallized glycerol tripalmitate standard. Sugars 

 identified: galactose, galactosamine, possibly trace of glucose. Sugars identified in 

 residues after lipid extraction: galactose, mannose, glucosamine, trace of glucose and 

 fucose, possibly trace of galactosamine. Mannose: galactose ratio approximately i :i 

 (contrast note i). 



(6) Fraction soluble in perchloric acid (Paul, 1958); RNA estimated by Ceriotti's (1955) 

 orcinol reaction using ribose as standard. 



(7) Fraction soluble in perchloric acid; DNA estimated by Ceriotti's indole reaction 

 (see Paul, 1958) using thymus nucleic acid (sodium salt) as standard. 



matter of considerable importance that Kandutsch (i960 and in 

 this volume) should have shown that his "enhancing" antigen 

 can be made soluble without serious loss of potency in the non- 

 ionic detergent Triton 100. (For all we know to the contrary, 

 Kandutsch' s enhancing antigen might sensitize if administered 

 in a way designed to reveal that capability.) In our experience, 

 antigenic sediment which has been briefly exposed to o- 5 per cent 



