TRANSPLANTATION ANTIGENS AND HAEMAGGLUTININS 



29 



Table I 

 Antibody titres obtained by the injection of CBA cells into SA mice 



Expt. 

 no. 



6 



7 

 8 



9 



10 



No. of 

 recipients 



10 



9 



10 

 10 

 10 



9 

 10 

 10 

 10 

 10 



Dosage of 



cells per Interval of 



injection per mouse injections 



( X 10^) in days 



Spleen cells 



(intraperitoneal) 



75 o 



70-36 7 



75-14-40 7 



100- 150- 150 - 140 7 



200-250-250-250 7 



Epidermal cells 



(intravenous) 



I o 



2-2 7 



2-2-2 7 



i-i-i-i 7 



i-i-i-i-i 7 



Serum taken 

 on days 

 after last 

 injection 



10 

 10 

 10 

 10 

 10 



10 

 10 

 10 

 10 

 10 



Red-cell 



antibody 



titre 



o 



1/64 



1/256 

 1/512 

 1/1024 



o 

 1/16 



1/32 

 1/64 

 1/128 



Table II 



Antibody titres obtained by the injection of CBA cell-free antigenic 

 extracts into SA mice 



BBM: Extracts prepared by technique of Billingham, Brent and Medawar (1958). 

 OC: Extracts prepared by technique of Oth and Castermans (1959). 

 LA: Extracts prepared by technique of Lejeune-Ledant and Albert (i960). 



extracts are injected by the intraperitoneal route in groups of 10 

 recipients at multiple intervals. The titre of haemagglutinins is 

 checked in the pooled serum of all the recipients of the same 

 group. The specificitity is again verified with isologous red 

 cells. As shown by the results given in Table II, all the types of 

 cell-free extracts elicit the formation of haemagglutinins. 



