SESSILE AND HUMORAL ANTIBODIES 



223 



Similar experiments were therefore performed in which immune- 

 cell-treated and control groups were challenged with AMT 3. 

 Adoptively transferred enhancing activity was likely to be more 

 easily detected in these circumstances. Fig. i shows the result of 

 one of these experiments, performed in C3H (J with 5 animals per 

 group. The three groups of mice treated intraperitoneally with 



15 DEAD 



Fig. I. Subcutaneous growth of AMT 3 in C3H cJ hosts. 



control group 



immune cell group 



Fraction denotes number of dead mice/total number in group. 

 Immune cell treated mice received pooled lymph node and 

 spleen cells from isogenic hosts sensitized five days previously 

 with AMT 3. Tumour size is plotted as average diameter in mm. 

 of group. 



doses of sensitized cells varying from 104 to 26 miUions showed so 

 Httle difference that they have been charted as a single group of 1 5 

 mice in the graph. All animals were injected subcutaneously with 

 ahquots of AMT 3 suspension prepared by mincing the tumour and 

 straining the resultant brei through a sieve. Subsequent tumour 

 growth was measured after depilation of the overlying skin. Two 

 diameters at right angles were recorded and the average diameter 

 in mm. of the group calculated. It can be seen that enhanced 



