81. ERYTHROCYTE AND HEMOGLOBIN VALUES: MAN 



HI Centrifuged at 2000 G or over (= 3000 rpm) for 10 min after attaining constant packed cell volume I 2] 

 /2/ Heparin or other isotonic anticoagulant. When anticoagulant = 2 mg K oxalate/ml blood, mean and 95% range 

 for Column C, Line 4 = 45(40-50); Line 5 = 41(36-45); Line 10, d = 35(30-40), 9 = 34(30-40); Line 14 = 82(70-94); 

 Line 15 = 86(74-97). [2] HI Specific gravity of RBC at 250C referred to water at 4°C [ 6] . /4/ Calculated from 

 volume (I6C) and specific gravity (20C). [ 6, j] /5/ Calculated from Hb content (13C). using 0.339 as % Fe in Hb 

 [2,8,9]. /6/ Use of radioactive chromium 51; other methods in essential agreement. [10] 111 M/ 15 phosphate 

 buffer at pH 7.4 [ 1 3] . /8/ Diffraction, or by 500 or more measurements with micrometer eyepiece. Range = range 

 of means. [1,14] /9/ Calculated from RBC volume (IbC) and dry diameter (29C) by formula t = V/)rr2 [1,2] . 

 /lO/ Calculated from RBC volume (16C) and dry thickness (31C) by formula A = 2irr (r + t) ( 2, h] . 



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