61. 



BLOOD LACTATE VENOUS LEVELS IN CONDITIONS OF REST, 

 EXERCISE, AND HYPERVENTILATION; MAN 



Data, except for Lines 4 and 7, were obtained on tungstic-acid filtrate via KMn04 oxidation to aldehyde, and 

 titration of bound aldehyde by iodine. Data for Line 4: through conversion to aldehyde by concentrated H2SO4, and 

 color formation with para-phenyl phenol. Data for Line 7: by oxidation with KMn04 and measured as CO2 mano- 

 metrically in Van Slyke apparatus. Values are expressed in mg/100 ml. Values in parentheses are ranges, esti- 

 mate "c" of the 95% range (cf Introduction). 



/!/ At sea level. /2/ At 22,000 ft. /3/ On 9 subjects on different days; day-to-day variations may reach t25% of 

 mean. /4/ On 3 subjects in good physical condition, walking 3.5-8.6 mph. /5/ Only the subject walking at 8.6 mph 

 showed rise in blood lactate (to 25.4 mg%). /6/ Increases in 3 subjects, walking 4.5-5.25 mph. /7/ Severity of 

 exercise not stated. /8/ Jogging at 6.48 mph. /9/ Running at 8.8 mph. /lO/ Samples taken 4-10 min sifter 440-yd 

 run by untrained subjects. Samples at 1-2 min show only 124 mg%, indicating that following strenuous exercise 

 blood lactate continues to rise for 3-6 min. /ll/ Hyperventilation to alveolar pCO^ of 11-15 mm Hg. /1 2/ Simu- 

 lated altitude reached without supplementary O2 in 1 hr (no acclimatization); approximately linear rise in blood 

 lactate starting at 10,000 ft. 



Contributor : Hegnauer, A. H. 



References : [ l] Cook, L. C, and Hurst, R. H.. J. Biol. Chera. 79:443, 1933. [2] Bock, A. V.. Dill, D. B., and 

 Edwards. H. T., J. Clin. Invest. nilTS, 1932. [3] Hummel, J. P., J. Biol. Chem. 180:1225, 1945. 

 [4] Friedemann, T. E., Haugen. G. E., and Kmieciak, T. C, ibid 157:67 3, 1945. "[Tl Laug, E. P., Am. J. 

 Physiol. 107:687, 1934. 



62. 



ARTERIO-VENOUS LACTATE DIFFERENCES IN CONDITIONS OF REST, 

 EXERCISE, AND HYPERVENTILATION: MAN 



Values are expressed in mg/100 ml. 

 Introduction). 



Values in parentheses are ranges, estimate "c" of the 95% range (cf 



HI A-V differences given as zero unless statistically significant, or greater than analytical error. /2/ Day-to-day 



variations in resting venous level may range from 7-25% of mean. /3/ Femoral artery. /4/ Femoral vein. 



/5/ Jugular bulb, lb/ Standing- running at full speed for 1 min. /7/ Blood samples taken within 3 min after exercise. 



181 Blood samples taken 5 rain after exercise. 191 1 mg difference may be real and indicate that removal rate at 5 



min exceeds production rate. /lO/ Hyperventilation to alveolar pCO^ of 11-15 mm Hg. /ll/ Radial artery. 



/12/ Arm vein. 



Contributor: Hegnauer, A. H. 



Referenc es: [1] Cook, L. C, and Hurst. R. H., J. Biol. Chem. 79:443, 1933. [2] Bock, A. V., Dill, D. B., and 

 Edwards, H. T., J. Clin. Invest. 11:775, 1932. 



60 



