152. O^ CONSUMPTION: FETAL TISSUES (Continued) 

 Part II: RAT (Concluded) 



With the exception of Lines 37 and 39, values in Lines 11-44 are based on Warburg manometric technique. Medium: 

 A = 0.8% NaCl, phosphate buffer, pH 7.4; B = serum + 0.025 M bicarbonate buffer + 0.2% glucose; C = serum + 

 0.025 M bicarbonate buffer + 0.011 M glucose; D = Krebs solution; E = Ringer-phosphate, pH 7.4. 



III O = cu mm O2 consumed per mg dry weight tissue per hr. /2/ Q^ 



cu mm lactic acid formed in O2 per mg 



N? 

 dry weight tissue per hr. /3/ Q^ = cu mm lactic acid formed in N^ per mg dry weight tissue per hr. /4/ QS = cu 



/5/ Membranes intact. /6/ Membranes 



ram acid (carbonic + lactic) formed per mg dry weight tissue per hr. 

 destroyed. /7/ Mediunn contained added glucose. 

 Contributor : Fitzgerald, L. R. 



References : [ l) Boell, E. J., and Nicholas, J. S.. J. Exp. Zool. 109:267. 1948. [2] Boell, 

 J. S., Science 90:411. 1939. [3] Negelein, E., "The Metabolism of Tumors," (ed. Warburg 

 Constable, 1930. [4] Kuomanomido, S., Biochem. Zschr. 193:31 5, 1928. [5] Mislivechkova, A 

 2:118. 1954. [6] Kleiber, M.. Cole. H. H.. and Smith. A. H., J. Cellul. Physiol. 22:167, 



1930. [8] Dickens. F.. and Greville, G. D. 



Part III: GUINEA PIG 



E. J., 

 O..). 



and Siraer, F., Biochem. J., Lond. 24:1301, 



and Nicholas 

 London: 

 .. Cesk. Morfol. 

 1943. [7l Dickens. F. 

 ibid 27:832, 1933. 



Values derived from blood-flow and blood-gas measurements. 



Con tribu tor: Fitzgerald, L. R. 



Reference : Bohr. C, Skand. Arch. Physiol.. Berl. ]^:413, 1900. 



Part IV: CHICK 



Section 1 

 Warburg manometric procedures on isolated embryos. Manometric determinations on intact embryos followed by 

 extensive calculations based on separate studies of membrane growth and respiration [ l] . Medium: A = intact egg 

 in air; B = intact egg in air and isolated tissues in Ringer-phosphate. pH 7.4; C = isolated embryo in Ringer- 

 phosphate. pH 7.2; D = isolated embryo in Krebs solution + 0.24%) glucose; E= isolated embryo in Ringer-phosphate. 

 pH 7.4; F = isolated embryo in Ringer-phosphate or Ringer-bicarbonate. pH 7.4. Where values are enclosed in 

 parentheses, glucose was added to the medium. 



300 



