122 W. M. Wallace, W. B. Weil and A. Taylor 



100 g. of protein plus ash. This is equivalent to the commonly 

 used reference standard of fat-free dry tissue (vide supra). 



Fig. 1 graphically presents the currently obtained data in 

 terms of grams of ash, protein and water per kilogram of fat- 

 free body. The grams of fat per kilogram of fat-free body are 

 shown to the right of the columns. It is evident that the 

 compositions of the fat-free bodies are essentially similar. The 

 relative proportions of water, ash and protein have not been 

 greatly modified by variation of the diet producing the growth 



GROUP PROT ASH 

 ASH PROT 



WEAN 5.0 2.0 



HPHE 5.6 1.8 



HPLE 6.3 



1.6 



LPHE 4.5 22 



LPLE 4.9 



2.0 



FRISKIES 4.8 2.1 



500 

 (g./kg. Fat Free Body Wecomt) 



Fig. 1. Ash, protein and water content calculated per 



kilogram of body weight for the six groups. Fat per 



kilogram of fat-free body is shown at the right. 



increment. Only if body fat were included would gross 

 variation occur. The young animals (WEAN) are relatively 

 low in ash and protein and high in water; with growth the 

 bodies acquired relatively more ash and protein than they 

 did water. The fat contents of the animals on the low 

 protein diets are significantly higher than they are on the high 

 protein. 



In Fig. 2 the absolute values for total fat-free body weight, 

 water, protein and ash for the five groups are shown as con- 

 trasted against the Friskie group as an arbitrary reference 



