Effect of Adrenal Steroids on Body Electrolytes 195 



Desaulles, p. a. (1958). In Aldosterone, ed. Muller, A. F., and 



O'Connor, C. M., p. 29. London: ChurchilL 

 Desaulles, P. A., and Meier, R. (1954). Unpublished data. 

 Desaulles, P. A., and Meier, R. (1956). Schweiz. med. Wschr., 86, 



1060. 

 Desaulles, P., Schuler, W., and Meier, R. (1955). Schweiz. med. 



Wschr., 85, 662. 

 DoRFMAN, R. I. (1949). Proc. Soc. exp. Biol., N.Y., 72, 395. 

 Gaunt, R., and Chart, J. J. (1958). Symposium on Homeostatic 



Mechanism, Brookhaven National Laboratory (in press). 

 Gaunt, R., Lloyd, C. W., and Chart, J. J. (1956). Colston Pap., 8, 233. 

 Johnson, B. B. (1954). Endocrinology, 54, 196. 

 Marcus, S., Romanoff, L. P., and Pincus, G. (1950). Endocrinology, 



50, 286. 

 McCance, R. a., and Widdowson, E. M. (1951). Proc. R. Soc, 138 B, 



115. 

 Meier, R., and Desaulles, P. A. (1956). Rev. iber. Endocr., 3, 565. 

 Olbrich, O., and Woodford-Williams, E. (1956). In Experimental 



Research on Ageing, ed. Verzar, F., p. 236. Basle: Birkhauser. 

 Schmidlin, J., Anner, G., Billeter, J.-R., and Wettstein, A. (1955). 



Experientia, 11, 365. 

 Schmidlin, J., Anner, G., Billeter, J.-R., Heusler, K., Ueber- 



WASSER, H., WiELAND, P., and Wettstein, A. (1957). Helv. chim. 



Acta, 40, 2291. 

 Singer, B. (1957). Endocrinology, 60, 420. 

 Stanbury, S. W., Gowenlock, A. H., and Mahler, R. F. (1958). In 



Aldosterone, ed. Muller, A. F., and O'Connor, C. M., p. 155. 



London: Churchill. 



DISCUSSION 



Adolph: Dr. Krecek, how do you account for what I take to be an 

 absence of water diuresis in rats at 23 days of age? Is it because they are 

 weaned early? Unweaned rats have a large water diuresis at this age. 



Kfecek : Water diuresis always occurs in rats of 23 days of age, but 

 during the first three hours after a water load there is a retention of one- 

 twentieth of the load. This figure was arrived at from balance tests, 

 being the difference between water load and water excretion. 



Heller : I am very pleased about the agreement between your findings 

 and ours, Dr. Kfecek. You use much the same technique as we did to 

 estimate the response of your animals to vasopressin, and you say that 

 you collect the urine for three hours after the injection. Did you have a 

 special reason for choosing this time interval? 



Kfecek: Yes, it was because the pattern of diuresis changes after 

 the administration of vasopressin, so that after three hours the excre- 

 tion of the water load is complete. 



Heller : For how long did your dose of vasopressin inhibit the water 

 diuresis of the adult animals which you used for comparison? 



Kfecek: When we give enough vasopressin for maximum diuresis we 



