532 BELANGER, ROBICHON, MIGICOVSKY, COPP, VINCENT 



The present various series of experiments cast some light on the 

 nature of basophiha, on the mineral-organic interdependence, and 

 also on the parathyroid-vitamin D-bone relationship. 



Materials and Techniques 



Animals 



Chicks. Twenty-four chicks were raised on low-calcium-no vita- 

 min D rachitogenic diet (Association of Official Agricultural Chem- 

 ists, 1940 ) from the time of hatching and distributed as follows : 



1. Six birds were given, after 1 week, a single oral dose of 1000 

 units vitamin D3. 



2. Six birds received the same treatment and also 3 daily doses 

 of 1 ml Para-Thor-Mone ( parathyroid extract, Lilly, 100 units ) intra- 

 peritoneally during the 2nd week. 



3. Six birds were administered Para-Thor-Mone as above, without 

 vitamin D. 



4. Six birds were maintained as rachitic controls. 



All birds were sacrificed on day 14, 24 hours after the adminis- 

 tration of tracer. The tibiae were roughly dissected and fixed in 10 

 per cent neutral formaldehyde for 48 hours. 



Rats. Twenty-four female Sprague-Dawley rats, of 150 to 200 

 gm body weight, were distributed and treated as follows: 



1. Six rats after approximately 10 days of pregnancy were given 

 3 daily doses of 1 ml Para-Thor-Mone and killed on the 4th day. 



2. Six nonpregnant rats were given 3 daily doses of 1 ml Para- 

 Thor-Mone and killed on the 4th day. 



3. Six rats of the same duration of pregnancy as those of group 1 

 but without Para-Thor-Mone were killed at the same time. 



4. Six nonpregnant rats without Para-Thor-Mone were also killed 

 as controls of group 2. 



Dogs — Para-Thor-Mone, Fourteen young dogs of both sexes 

 were treated as follows shortly after weaning: 



1. One animal was injected with 100 units of Para-Thor-Mone in- 

 tramuscularly for 3 successive days and killed on the 4th day. 



