RESORPTION WITHOUT OSTEOCLASTS (OSTEOLYSIS) 535 



Pieces of these specimens were then embedded in acryhc resin for 

 microradiography (Belanger et ah, 1963). Comparable pieces were 

 deminerahzed in EDTA or in nitric ethanol (nitric acid 5 per cent 

 in 70 per cent ethanol) and then embedded in paraffin. 



Alpharadiographs (Belanger and Belanger, 1959) of 10-micron 

 nonradioactive demineralized sections were obtained on Eastman 

 Kodak spectroscopic plates, type V-O and type 649-0. Alpharadio- 

 graphs of 12-micron mineralized sections were recorded on Di-Noc 

 Contrast II plates (Di-Noc Chemical Arts Ltd., Honeoye, New 

 York). 



Adjacent mineralized sections were also submitted to x-ray micro- 

 radiography with a Philips 5-kv apparatus and the results were 

 recorded on Eastman Kodak film type 649-0. 



Series of mineralized and demineralized sections of the tibiae were 

 prepared for phase contrast microscopy in Permount (Fisher), 

 without stain. 



Series of demineralized sections were stained by the Masson tri- 

 chrome stain with light green (Masson, 1929), with toluidine blue 

 (Belanger and Hartnett, 1960), by the Hale iron adsorption method 

 for acid mucopolysaccharides (Hale, 1946), by the Hotchkiss-Mc- 

 Manus periodic acid-Schilf method (Gomori, 1952) for neutral 

 polysaccharides (Hooghwinkel and Smits, 1957; Leblond et ah, 

 1957), and h\ the combined Alcian blue-PAS stain of Mowry 

 (1956). 



Observations 



Chicks 



Staining. Bone sections stained by the Masson trichrome show 

 areas which take up the red dyes and other sites which are green 

 acceptors. The material of the present series has revealed definite 

 patterns of staining apparently related to treatment. The trabecular 

 matrix and also the characteristically thick periosteum of rachitic 

 birds were stained almost exclusively green ( Fig. 1 ) . This statement 

 applies at least to matrix formed during the nutritional experiment. 



In birds treated with Para-Thor-Mone (Fig. 2), the trabeculae 

 were thicker and mostly green. However, the more mature trabeculae 



