536 be:langer, robichon, migicovsky, copp, vincent 



located on the marrow side of the diaphysis showed progressively 

 extensive red patches ( Fig. 2 ) . 



The chicks which received vitamin D showed a predominance of 

 red staining, beginning early, at the site of growth (Fig. 3), i.e. close 

 to the periosteum. Those which received both vitamin D and Para- 

 Thor-Mone produced thin trabeculae which were stained green close 

 to the periosteum, then red. The more centrally located ones were 

 green at the immediate border (immature bone or osteoid), then 

 red, then green again in the mid-trabecular region ( Fig. 4 ) . 



Acid mucopolysaccharides. The osteocytes particularly in all 

 chicks which received vitamin D were different in size and also in 

 staining properties (Figs. 11 and 12). Close to the periphery of the 

 trabecula, the cells and lacunae were small and poorly stained. They 

 seemed to enlarge progressively toward a maximum size in the 

 center of the trabeculae, where the cytoplasm of the osteocytes 

 exhibited toluidine blue metachromasia, azurophilia (Fig. 11), and 

 a positive Hale reaction ( Fig. 12 ) . The matrix immediately surround- 

 ing the lacuna was also metachromatically stained (Fig. 11). The 

 adjacent matrix did not show metachromasia, but basophilia (Fig. 

 11) progressively decreasing away from clusters of large osteocytes 

 (Fig. 11). The Hale reaction was localized to the cytoplasm and to 

 the processes of the large osteocytes ( Fig. 12 ) . 



Tissues which were demineralized in nitric-ethanol apparently 

 retained larger amounts of the metachromatic, Hale-positive, and 

 alcianophilic material than tissues demineralized in EDTA. In both 

 cases, however, the localizations were similar. 



In chicks treated with Fara-Thor-Mone and vitamin D, several of 

 the enlarged lacunae located in the mid-trabecular region either were 



Figs. 1 to 4. Masson-stained EDTA-demineralized sections of mid diaphy- 

 sis of 3-week-old chicks. ( X 88.) 



Fig. 1. On rachitogenic diet. 



Fig. 2. On rachitogenic diet and Para-Thor-Mone treatment. 



Fig. 3. On rachitogenic diet and vitamin D treatment. 



Fig. 4. On rachitogenic diet and vitamin D— Para-Thor-Mone treatment. 



The periosteum (P) is in the lower part of the pictures. The marrow side 

 ( M ) is uppermost. 



