592 DOWSE, NEUMAN, LANE, AND NEUMAN 



tissue are distinctly different from those of most soft tissue and must 

 be representative, in a qualitative way, of new bone or bone-forming 

 tissue. 



Results and Discussion 



Because of the many metabolic aspects to be covered, a discussion 

 of each is presented along with the results. 



Oxidation 



The rate of oxygen consumption of calvaria in phosphate medium 

 containing no added substrate is presented in Fig. lA. Despite the 



HOURS 



HOURS 



Fig. 1. Oxygen consumption of calvaria incubated in phosphate medium 

 in vitro. Qq.-, expressed as /imoles per gm wet weight. A, data showing the 

 continued hnear oxygen consumption over an 8-hour period. B, data showing 

 the enhanced oxygen consumption from the addition of 10 mM succinate (S) 

 and subsequent inhibition by malonate (M); numbers indicate concentration 

 in mM. C, data showing the effects of additions of the inhibitor fluoroacetate; 

 numbers indicate its concentration in mM. 



