PARATHYROID HORMONE AND RAT CALVARIA METABOLISM 



603 



150- 



100- 



4 6 



HOURS 



Q, 



•/CONTROL 4 



B 



I 

 LOG DOSE 



Fig. 3. The effects of paiathxroid hormone on lactate production by 

 calvaria. A, Qr„ /xmoles per gm wet weight over 8 hours in the presence (5 

 units per ml) and absence of parathyroid hormone. B, the relation between 

 parathyroid hormone concentration and lactate production; Aq^ represents the 

 difference, in /(.moles gm/hr., between paired control incubations and the 

 indicated dosage of parathyroid hormone expressed as log units per ml + 1. 

 Thus, the highest dose corresponded to 45 units per ml of Rasmussen polypep- 

 tide. 



despite a Pasteur effect, a high rate of aerobic glycolysis (Laskin 

 and Engel, 1956; Borle et al, 1960a; Cohn and Forscher, 1961). 

 Though these studies represent the first reported (Neuman and 

 Dowse, 1961) metabolic effects in bone resulting from purified para- 

 thyroid hormone added in vitro, the effects were similar to those 

 observed in metaphvses from animals given massive doses of hor- 

 mone parenterally (Laskin and Engel, 1956; Borle et al, 1960a; 

 Cohn and Forscher, 1961). 



In view of the rather profound physiological effects of parathy- 

 roidectom\' in most species, the observed metabolic changes from 

 excess hormone seem rather minor. In fact, the number of parameters 

 which are unaffected is remarkable and suggestive in itself: no 

 change in Qo.^, no change in glucose uptake, no change in citrate 

 production, no change in lactate production anaerobically. 



One gains the impression that any hormonal effects on carbohy- 



