006 DOWSE, NEUMAN, LANE, AND NEUMAN 



Though control studies indicated the operation of the Krebs cvcle 

 as the principal oxidatixe pathwa\-, the most striking metabolic 

 properties of calvaria were: almost exclusive utilization of glucose 

 (or gh'cogen), a high and medium-dependent ghcolytic rate, a high 

 aerobic glycolysis (incomplete Pasteur mechanism), and a low but 

 medium-independent oxidative rate. 



Purified parathyroid polypeptide added to the medium showed 

 relatively little influence on metabolic activit\ . There were no sig- 

 nificant changes in citrate production or utilization, glucose uptake, 

 Qo.,, or levels of Ca++ or inorganic phosphate in the medium. The 

 only significant finding was an increased production of lactate, and 

 tliis only under aerobic conditions. 



The impression is gained that the action of the hormone on this 

 tissue under conditions in vitro may be on some system other than 

 glucose metabolism, and that the metabolic changes, though definite, 

 are small and indirect in character. Arguments are presented, along 

 with preliminary evidence, that the hormone may influence the 

 transport of phosphate in and/or out of the cell. 



References 



Albright, F., and Reifenstein, E. C, Jr. 1948. The Parathyroid Glands and 



Metabolic Bone Disease. Williams and Wilkins Co., Baltimore, Md. 

 Barker, S. B., and Summerson, W. H. 1941. The colorimetric determina- 

 tion of lactic acid in biological material. /. Biol. Chem., 1-38, .535- 



554. 

 Beaulieu, M. M. 1953. X-rays and the citric acid of the bones. Bull. soc. 



chim. biol, 35, 491-500. 

 Borle, A. B., Keutmann, H. T., and Neuman, W. F. 1963. The role of 



parathyroid hormone in phosphate transport across the rat duodenum. 



A7n. J. Physiol, 204, 705-709. 

 Borle, A. B., Nichols, N., and Nichols, G., Jr. 1960fl. Metabolic studies of 



bone in vitro. I. Normal bone. /. Biol. Chem., 235, 1206-1210. 

 Borle, A. B., Nichols, N., and Nichols, G., Jr. 1960/;. Metabolic studies of 



bone in vitro. II. The metabolic patterns of accretion and resorption. 



/. Biol. Chem., 2.35, 1211-1214. 

 Chen, P. S., Jr., and Toribara, T. Y. 1953. Determination of calcium in 



biological material by flame photometry. Anal. Chem., 25, 1642-1644. 

 Chen, P. S., Jr., Toribara, T. Y., and Warner, H. 1956. Microdetermina- 



tion of phosphorus. Anal. Chem., 28, 1756-1758. 



