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Some Chemical Factors Influencing 

 Bone Resorption in Tissue Culture 



PAUL GOLDHABER, Department of Oral Histopathology and Perio- 

 dontology, Harvard School of Dental Medicine, Boston, Massachusetts 



DESPITE the pioneering work of Gaillard (1955fl, 1955/?, 1957, 

 1961«, 1961&), who demonstrated a direct effect of parathyroid 

 tissue or extract on bone in tissue culture, others, inchiding ourselves, 

 were not able to obtain similar evidence until most recently. In the 

 course of our work, however, we did find a method of producing 

 bone resorption routinely in tissue culture, without adding para- 

 thyroid extract to our system, thereby permitting investigation of 

 some of the morphological and biochemical changes related to bone 

 resorption per se. Of particular interest to us was the development of 

 a tissue culture system which would enable us to study the mecha- 

 nism of action of potential stimulators or inhibitors of resorption. 

 The purpose of the present paper is to describe the development of 

 such a system and some of the significant chemical factors which 

 have been found to exert a pronounced effect on the phenomenon 

 of bone resorption. 



Observations 



Regulation of Bone Resorption by Oxygen Tension 



In our earlier experiments ( Goldhaber, 1958, 1960 ) it was found 

 that bone resorption could be induced within young mouse calvaria 



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