BONE RESORPTION IN TISSUE CULTURE 623 



EFFECT OF DIFFERENT CONCENTRATIONS OF 

 PARATHYROID EXTRACT (LILLY) ON CALCIUM 



LEVEL IN TISSUE CULTURE MEDIA 



I6r 



15 



14- 



13- 



3 

 O 



< 



o 



12- 



10- 



9- 



U/ML 



0.5— ^^ 



./\ 



'••^. 



(C 



0.1 — .•'*' 

 0.0 5 -^y^ 



o.oi-\ X 



00 - ^ ^ - ^>v^<r- \ \ 



;ONTROL)V' ^ '^ n\ N 



"■ -^ 



6 8 



DAYS 



10 



12 



Fig. 27. Graph showing calcium levels in media removed from same ex- 

 periment as shown in Fig. 26. Note that the increased calcium levels obtained 

 with graded doses of parathyroid extract reflect the bone resorption observed 

 morphologically. 



tubes being gassed with 50 per cent O2) led us to reinvestigate the 

 question of citrate versus lactate production by resorbing calvaria. 

 This work was done in collaboration with Dr. George R. Martin's 

 laboratory and will be published elsewhere (Mecca et al, 1963). 



