46 



A. B. BORLE 



bone, kidney and intestine (Borle ct ai, 1960; Egawa and Neuman, 1963; Borle 

 et ai, 1963). In HeLa cells, a) PTH increased lactate production ZS-S^/o from 

 32.2 ± 1.98 to 41.4 ± 1.16 //g/hr/10« cells (p< 0.001); b) PTH increased the cellular 







-^^•^ '^: 





r^: 



Pi 21''/o; c) PTH increased ^-P incorporation into the lipid fraction of the cells 63Vo 

 in one hour (relative specific activity 4.25 XIO"^ in control vs. 6.93 XIO"^ in the 

 experimental group). In monkey kidney cells, PTH did not increase lactate produc- 

 tion but increased the total cellular phosphate 30^/0 from 26.1 to 34.0 //g P/mg cell 

 protein (p<C 0.001). Table 1 presents a summary of these changes and a comparison 



Table 1. Metabolic effects of PTH 



Lactate Coll P 



Production I'ptake 



3 21. 



lipid 



HeLa Cells . . . . 

 Monkey kidney cells 

 Bone slices 1' - . . . 

 Kidney slices - . . . 

 Intestine-^ 



(LE Ct al., 1960. 



+ 30% I (+ 21%) 



I + 30% 



+ 34% I + 38% 



- I + 26% 



+ 36%, I + 28% 



Egawa and Neuman, 1963. 



+ 63°o 



+ 34% 



» Borle et al, 1963. 



with the results previously obtained in bone, kidney and intestine. It appears from 

 these studies, that PTH may enhance aerobic glycolysis, phosphate transport into 

 the cell and probably the turnover of phospholipids. In view of the morphological 

 observations reported above, it is of interest that very similar metabolic changes have 

 been reported in phagocytosing leucocytes (Sbarra and Karnovsky, 1959, 1960). 



