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Correlation between Morphological, Biochemical, and Biophysical 

 Effects of Parathyroid Hormone on Cell Membranes 



A. B. Borle 



Department of Physiology, University of Pittsburgh School of Medicine, 

 Pittsburgh, Pa., U. S. A. 



We have previously reported that parathyroid hormone (PTH) added to HeLa 

 cells decreased cellular adhesion to glass, produced cell clumping (increased mutual 

 adhesion of the cells) as well as the appearance of blebs and microvilli of the cell 

 membrane (Borle and Neuman, 1965). Monkey kidney cells seem to respond to 

 PTH as well. One unit of PTH/ml of medium produced a large number of vacuoles 

 in the cytoplasm and an enlargement and swelling of the Golgi apparatus (Fig. 1 and 

 2). Sequential observations suggest that the vacuoles are formed by pinocytosis. 



Fig. 1. M0IlkL■^ kl 



Metabolic Studies: Both of these cell strains were incubated in Krebs-Ringer 

 bicarbonate buifer to determine whether the morphological modifications produced 

 by PTH would be associated with metabolic changes comparable to those observed in 



