42 



^ %'• r V. 



J. S. Greenspan, H. J. J. Blackwood 



• f - 



•'^'Ife-.. 



'»- . .'* 



Fig. 2. Cells within the mineralising '/one of the c.iitilagc showing granular stai 

 fixed 8 ,(( cryostat section, AS.TR phosphate, fast dark blue R salt, pH 5.5, nn 



ning tor acid phosphatase. Un- 

 :ubation time 15 minutes. 400 





A...\ 



\ >-^ 



^ 



•^ 



Fig. 3. VON Koss.'i reaction showing the level of mineralisation in the h> pertropluc '/one of the cartilage. Unfixed 

 S (( crvostat section. > 160 



