176 



R. Steendijk, J. JowsEY, A. VAN DEN HooFF, H. K. L. Nielsen 



the osteone (Fig. 4). Non-mineralised osteoid, at the inner aspects of growing 

 Haversian systems, did not take up these stains and did not exhibit an abnormal 

 pattern under phase-contrast. The van Gieson-, Mallory-, PAS-, and Sudan-black- 



Fig. 1. Azure II — picric acid stained, 20 /< thick section from decalcified bone from the fibula of a 22-year- 

 old man with primary vitamin D-rcsist.inr rickets (X 190) 



t 



Fig. 2. Microradiograph from a 100 n thick undecilcilicd cross 



• ;,( 



Fig. I. ( •; 100) 



stains failed to reveal anything abnormal. A slight affinity for alcian blue at these 

 sites was observed only very occasionally; with haematoxylin the perilacunar regions 

 were discernible with difficulty and inconsistently by a slightly darker hue. Prior 



