Microradiographic and Histological Observations in Primary Vitamin D-resistant Rickets 177 



digestion of the sections with pepsin or trypsin did not interfere with the pattern as 

 revealed by azure II; the acrolein-Schiff reaction for protein (van Duyn, 1961) failed 

 to demonstrate the lesion. 





j|*'^%' 



\ 



Fig. 3. Azure II — picric acid stained, 6 a thick section from the same bone as in Fig. I. (,\ 12C0) 





'^^^•:S'^ 



'■*rr"^ 



0^ 





^ ^ 



Fig. 4. Phase-contrast photomurogi iph ot an unstained, 10 n thick section irom the s.ime bone as in Fig. 1. 



( < 1200) 



Discussion 



The findings described above favour the hypothesis that a structural change rather 

 than a chemically defined abnormality of the matrix is responsible for the observed 

 affinity for azure II. As far as the authors know, this particular type of lesion has 



3'''' Europ. Symp. on Cal. Tissues 



