Preliminarv Studies of the Ultracentrifugal and Free Zone Electrophoresis 



273 



We do know that its X-ray diffraction pattern reveals that embryo-enamel 

 matrix is in a cross-/i-configuration (Glimcher et al., 1961 a). Recently it has been 

 shown that soluble enamel protein reconstituted from solution also has a well defined 

 cross-/?-configuration (Bonar et al., in press). The amino acid composition of enamel 

 matrix is characterized by large amounts of proline, glutamic acid and histidine 

 (Glimcher et al., 1961 b). More recently it was shown that decalcified bovine embryo 

 enamel matrix may be solubilized by cold neutral buffer and that essentially the 

 same amino acid composition prevails (Glimcher et al., 1964). 



This paper presents some of the solution characteristics of bovine premolar- 

 embryo enamel protein. 



Experimental and results 



In sedimentation velocity experiments the number of schleiren peaks, their 

 apparent sedimentation coefficients, and the distribution of the protein between the 



Fig. 1. Synthetic boundary sedimentation velocity experiments at 4°, 0.1 M salt, pH 7.7. The boundary was 

 formed about three-fourths from the bottom of the cell which is on the right. Sedimentation is in the direction 

 of the arrow, a) 1.4''/o protein solution, 120 min., 42, 040 RPM, phase angle 60'=, b) 0.7''/o protein solution, 

 50 min., 59, 780 RPM, phase angle 60°, c) 0.35''/o protein solution, 50 min., 59, 780 RPM, phase angle 50-', 

 d) 0.175''/o protein solution, 33 min., 59, 780 RPM, phase angle 40°, e) 1.4''/o protein solution, 150 min., 59, 

 780 RPM, then 130 min. at 42, 040 RPM, phase angle 50- 



3'''^ Europ. Symp. on Cal. Tissues 



