PSEUDOHEMOGLOBIN 487 



tive, probably ferricholeglobin cyanide, while Barkan's compound was the 

 corresponding denatured compound. 



Kiese and Kaeske {1527), while confirming the great spectroscopic 

 similarity between pseudohemoglobin (or, as they call it, verdoglobin- 

 CN) and choleglobin (or, as they call it, verdoglobin- A), found 

 differences in the position of the absorption band of the hydrazine 

 and particularly the carboxy hydrazine hemochromes (Table III). 



TABLE III 



Absorption Spectra of Choleheme and Pseudoheme Compounds" 



" According to Kiese and Kaeske (1527). 



Holden (1319) obtained a "pseudoheraatin" which resembled cru- 

 oratin (see below), except that the absorption bands of its compounds 

 were 10-11 m/z closer to the infrared. Carboxypseudoheme showed 

 a moderately strong Soret band (cmM = 50). Pseudohemiglobin 

 cyanide, produced by the action of hydrogen peroxide and buffered 

 cyanide on hemoglobin, has an absorption band at 590-600 m/Lt.'f 



Kiese and Kaeske used high concentrations of both peroxide and buffered 

 cyanide; a large part of the product was denatured, but the experiments were 

 carried out with the part which remained in solution after dialysis, and which 

 was almost free from unaltered hemoglobin. Liebecq {1738a) separated the 



* Liebecq and co-workers (1738c,1738d) have recently come to the same conclusions 

 as Lemberg and co-workers (cf. above). Their observation that the cyanide is removed 

 on reduction agrees with our findings and does not contradict the observations of Lem- 

 berg and co-workers (1709) that the hemochrome is able to combine with cyanide. 

 It is well known that hemtglobin cyanide dissociates on reduction, while hemochrome 

 forms a cyanide compound. 



According to the Belgian workers, pseudohemoglobin (as well as choleglobin) con- 

 tains a small admixture of an oxyporphyrin compound, to which the absorption band 

 at 670-680 m^ of cyanide-free pseudohemoglobin and of ferricholeglobin is ascribed. 

 Evidence for partial conversion of pseudohemoglobin into a monoazahemochrome 

 by ammonia was obtained. 



The contradictory findings with regard to the Soret band, the liberation of iron, 

 and the yield of bile pigment — compare Liebecq's findings with those of Kiese 

 (1526a) and also the next subsection — and recent observations of Lemberg and Calla- 

 ghan (1694) suggest that slight variations of the same method may lead to different 

 compoimds, as in the instance of sulf hemoglobin (see below). 



