CONTENTS XVll 



6.2.2 Hb + 02->Hb02 279 



6.2.3 CO + Hb^HbCO 281 



6.2.4 HbCO -> CO + Hb 281 



6.2.5 CO + Hb02 ^ HbCO + O2 282 



6.2.6 HbCO + O2 -> Hb02 + CO 283 



6.2.7 Kinetics of Reactions within the Erythrocyte 284 



6.2.8 Kinetics of Myohemoglobin Reactions 284 



6.3 Kinetics of Other Reactions 285 



7" Interpretation of Kinetic Data 285 



7.1 Relation to Theories of Equilibrium 285 



7.1.1 Anomalous Support for Hufner's Theory 285 



7.1.2 Reactivity of Freshly Reduced Hemoglobin 286 



7.2 Comparison of Kinetics of Myohemoglobin and Hemoglobin 287 



7.3 Differences between the Kinetics of Oxygen and Carbon 

 Monoxide Reactions 287 



7.3.1 Effect of pH 287 



7.3.2 Differences in Heats and Entropies of Reaction .. . 288 



7.3.3 Spectroscopic Differences 289 



8 Structural Basis of Heme-Heme Interaction 291 



8.1 Classification of Hemoglobin Equilibria 291 



8.2 Pathway of the Interaction 292 



8.3 Hemoglobins of Different Molecular Weight 295 



9 Determination of Hemoglobin 295 



9.1 Estimation of Hemoglobin for Clinical Purposes 295 



9.1.1 Specificity 295 



9.1.2 Errors of Technique and Standardization 296 



9.2 Manometric and Gasometric Methods 297 



9.3 Spectrophotometric, Photoelectric, and Spectrocolorimetric 

 Methods 298 



9.4 Colorimetric Methods 300 



9.5 Other Methods and Estimation of Hemoglobin in Plasma, 

 Urine, and Tissues 301 



9.6 Special Estimations 302 



VII. Comparative Biochemistry of Hemoglobins 305 



1 Introduction 305 



2 Biological Distribution 306 



3 Chemical Basis of Spectroscopic Differences 308 



3.1 Classification 308 



3.2 Chlorocruorin 308 



3.3 Erythrocruorin 309 



3.4 Hemoglobin and Myohemoglobin 309 



3.5 Influence of Protein on the Spectrum 310 



4 Molecular Weight Classes of Respiratory Pigment 311 



4.1 Classification 311 



4.2 Extracellular Oxygen Carriers 311 



4.3 Intracellular Oxygen Carriers 312 



4.4 Dissociation of Erythrocruorins 312 



4.5 Physiological Implication of Particle Size 312 



5 Protein Differences 314 



5.1 Lsoelectric Points 314 



5.2 Amino Acid Analysis 315 



