HEMOGLOBIN EQUILIBRIA 



267 



Altschul and Hogness U^), Wyman and Ingalls (3136), and Coryell 

 U98) discuss procedures which assist in the latter process. 



(t 0/2 0.4 0.6 0.8 1.0 



DEGREE OF OXYGENATION 



y 



Fig. 7. Relative concentrations of intermediates between Hb4 and Hb4(02)4 (after 

 Coryell, Pauling, and Dobson, 500). The curve for /rfl«s-Hb 4(02)2 is not given; it 

 has 1/24 the height of II throughout. 



5.1.6. Present Status of the Equilibrium Problem. Pauling's equation 

 was based on the behavior of horse hemoglobin which liad been purified by 

 the procedure of Ferry and Green (747) and which had been investigated at 

 ionic strengths from n = 0.'^ to ^ = 0.4. While it fitted these data ade- 

 quately, difficulties arise when it is applied to other data. Thus Altschul and 

 Hogness (44-) reinvestigated the dissociation of Hb02 and took into account 

 the specific ionic eflfect, ionic strength, and pK. In addition, they purified 



