CHEMICAL BASIS OF SPECTROSCOPIC DIFFERENCES 309 



Spirographis porphyrin. Its structure has been fully elucidated by 

 the work of Warburg (2954.,2957), Fischer (880), and their co-workers. 

 It is a type IX porphyrin, differing from protoporphyrin in that one 

 vinyl group is replaced by formyl (cf. Chapter III). 



TABLE II 



Spectra of Chlorocruorin Derivatives" 



Derivative" Band position, mt/' 



Ch02 60i, 560 1 , , . , , . , V 



ChCO 600,557/^"^^"^"^"^^^'^^^^) 



Ch 574 (broad band) 



Ch hemochrome 569, 533 1 , , , i , i , n 



^.r ^1.1 1 .^„ > (sharp-banded hemochrome spectra) 



CN-Ch hemochrome -573, 538 J ^ f ^ 



Ch+ 604, 569 (both bands weak) 



ChOH Does not exist (?) 



" According to Fox (931). 



* Ch = chlorocruorin; Ch+ = ferrichlorocruorin; and ChOH = ferrichlorocruorin 



hydroxide (alkaline metachlorocruorin). 

 <^ The strongest bands are italicized. 



3.3. Erythrocruorin 



The spectrum of the erythrocruorins is similar in character to that 

 of the hemoglobins, the difference in band position being attributed 

 to the proteins. Salomon {21^23) has prepared mesoporphyrin IX 

 from the intracellular erythrocruorin of Glycera while Kirrmann 

 (1539) has shown that the prosthetic group of the pigment from 

 Chironomus is also type IX. 



The erythrocruorins differ from the hemoglobins in at least two 

 reactions involving the prosthetic group. Vies (2895) has shown 

 spectroscopically that the acid and alkaline forms of the oxidized 

 pigment, ferriery throcruorin and ferrierythrocruorin hydroxide, resem- 

 ble acid and alkaline hematins, rather than hemiglobin and hemt- 

 globin hydroxide; while Salomon found that Lumbricus erythro- 

 cruorin was unable to undergo a coupled oxidation with ascorbic acid 

 in the same way as does hemoglobin (cf. Chapter X). 



3.4. Hemoglobin and Myohemoglobin 



So far, type IX protohemin is the only one prepared from mam- 

 malian hemoglobin, and up to the present time no one has reported 

 spectra from the blood of any of the vertebrate species which would 



