Ultraviolet protecting 

 filter (Leitz #8574A) 



_^SIide 



J 



Ultraviolet filter 

 (Corning #584t428) 



Mecury lamp 



Figure 11-29. Schematic Diagram Showing the Arrangement of Micro- 

 scopic Components for the Study of Ceils and Tissues by Fluorescent Light. 

 (From Popper, H. and Szanto, P. B., 1950. "Fluorescence Microscopy," in 

 Jones, R. M. (Ed.), "McClung's Handbook of Microscopical Technique," 

 3rd ed., Paul B. Hoeber, Inc., Harper & Brothers. New York, N. Y., Fig. 

 130, p. 679.) 



ard microscope lenses can be used. The method most often employed in 

 fluorescence microscopy is the crossed filter technique (Figure 11-29). 

 A source filter that transmits only the ultraviolet radiation to be absorbed 

 by the specimen is positioned in front of the light source (mercury vapor 

 or carbon arc lamp). A complementary filter is mounted in the eyepiece 

 or body tube of the microscope for visual observation and photography. 



SURVEY OF CYTOLOGICAL TECHNIQUES / 251 



