THE PITUITARY BODY 



separate "A" and "B" principles; oestrus, however, does not 

 necessarily signify follicular growth and maturation. ■''' 



The assay of prolan in immature male mice and rats. — The 

 use of male animals for the assay of prolan was proposed by 

 Borst and Gostimirovic, and Brouha and Simmonet in 1930. 

 As was explained above, this is best accomplished by de- 



FiG. 41. — The relationship between the intravenous dose of one preparation of 

 prolan and ovulation in the rabbit. Each point (except two) represents a group of 

 more than twenty rabbits. The percentage of animals in which ovulation was ob- 

 served is indicated along the ordinate. From Hill, Parkes, and White (1934). 



termining the indirect effects on the accessory organs (e.g., 

 seminal vesicles) following the stimulation of the interstitial 

 cells. However, no one has seriously investigated the rela- 

 tionship between response and dose. 



The assay oj prolan in female rabbits. — The female rabbit, 

 like the female mouse and rat, has been extensively used for 



3° Also see Aschheim, Ehrhardt, Zondek (1929); Brouha and Simmonet (1930); 

 Wallen-Lawrence and van Dyke (1931); De Jongh and Kober, Katzman and Doisy 

 (1933); Reiprich, Rowe and others (1934); Nelson and Overholser (1935). 



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