GONADOTROPIC SUBSTANCES 



however, regressed rapidly and did not secrete progesterone. 

 In ovarian transplants in the anterior chamber of the eye, the 

 injection of prolan produced follicular hemorrhage as well as 

 follicular growth (Allen and Priest, 1932; also see Spirito, 



1933)- 



There is good evidence that the corpora lutea formed after 

 the injection of prolan secrete progesterone like normal cor- 

 pora lutea whether or not ovulation has also occurred. 

 Pseudopregnancy may either be initiated or prolonged with 

 the development of breast changes and the characteristic pro- 

 gravid uterus. McPhail (1933) injected 5 cc. of pregnancy- 

 urine intravenously into rabbits every 10 days. After each in- 

 jection ovulation followed and the animals remained pseudo- 

 pregnant during the period of observation (5 weeks). The 

 progestational changes in the uterus, however, seemed more 

 pronounced after 4 weeks than after 5 weeks. Siegmund 

 (1930), Winter (1931), and Robson (1932) have shown that 

 posterior pituitary extract either has no effect or inhibits the 

 movements of the isolated uterus of the doe rendered pseudo- 

 pregnant by the injection of prolan. Knaus had previously 

 shown that *'pituitrin-insensitivity" of the uterus could be 

 demonstrated in pregnant does during the period of active 

 corpus luteum secretion (see chap. xi). The uterus in situ 

 was found by Reynolds (1932) to become quiescent after the 

 injection of prolan. This change, unlike that just described, 

 appeared not to depend on indirect effects due to the internal 

 secretion of the corpus luteum — for it appeared (i) if only 

 follicular growth was produced, and (2) in ovariectomized 

 animals in which uterine motility had been increased by the 

 injection of oestrone. "Hormonal sterilization" was pro- 

 duced in rabbits by Reiprich (1934), who found that the 

 period of sterility was roughly 3 weeks after the administra- 

 tion of 2,000 rat-units of prolan, and might be prolonged to a 

 year, particularly after larger doses. Reiprich attributed the 

 sterility to a persistence of corpora lutea. Rosenblatt, Hal- 



[193] 



