THE PITUITARY BODY 



ber, and Pruszczynski (1932), however, considered that re- 

 peated large doses of prolan caused ovarian damage similar to 

 that caused by X-ray treatment. Rosahn, Greene, and Hu 

 (1934) injected prolan into female rabbits of low fertility 2 

 hours before to 24 hours after mating. Fertility was in- 

 creased. In the authors' opinion this was because ovulation 

 occurred more frequently; however, the effect might have 

 been equally well attributed to increased corpus luteum se- 

 cretion and consequent facilitation of implantation. 



Padoutcheva and others (1934) produced pregnancy in 

 rabbits by artificial insemination (vagina or uterus) at about 

 the time of ovulation induced by prolan. 



Martins and Fabiao (1930), Snyder and Wislocki (1931), 

 Hill and Parkes (1932), Jares (1932), and Wislocki and Good- 

 man (1934) have all produced ovulation in the pregnant doe. 

 The course of pregnancy usually was not altered as a result of 

 the formation of new corpora lutea; however, Hill and Parkes 

 believed that the latter sometimes adversely affected the cor- 

 pora lutea of pregnancy. Jares was of the opinion that the 

 ovulatory dose had to be increased if functioning corpora 

 lutea were present, but did not offer convincing evidence in 

 favor of this view. 



Prolan will still cause ovulation after injection into rabbits 

 hypophysectomized only a short time before, as was shown 

 by Hill and Parkes (1931) and White and Leonard (1933). 

 The latter were of the opinion that the dose had to be in- 

 creased about 50 per cent. Hinsey and Markee (1933) in- 

 jected large doses of pregnancy-urine intravenously into rab 

 bits after the removal of the cerebral hemispheres, dien- 

 cephalon, and hypophysis. They found that the size of the 

 rabbits and the interval between operation and injection af- 

 fected the ovarian response. In large does, ovulation occurred 

 less frequently if more than 3 hours elapsed between opera- 

 tion and injection. No ovulation was observed in smaller 

 does (2.0-2.3 kg-) which received prolan 5-40 minutes after 



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