1^6 



from a süßerer from tuberculosis, the other from a non- 

 sufferer, is equal, absolute checking of fibrine-transformation 

 by tuberculine appears with the first serum in a weaker 

 dilution than with the second. If the fibrolytic power of the 

 2 sera is not equal, but e.g. that of the second much stronger 

 than that of the first, the dilution for the absolute checking of 

 the fibrine transformation need not be weaker for the first 

 serum than for the second. 



I will illustrate this by some experiments. 



Serum A is obtained from a female sufferer from phthisis in 

 the first stage with a temperature up to 38° C. Serum B 

 originates from a female sufferer from ulcus ventriculi. Both 

 sera are respecteivly diluted 50, 100, 200 and 400 times with 

 Na. CI. solution. To all dilutions of both sera pieces of fibrine 

 of the same size, the same date and the same colour-strength 

 are added. The fibrine is fresh, 2 days old. 



The following mixtures are prepared : 



1. 2 cM^. NaCl. + 0,5 M^. Serum A (10 X diluted) + fibrine. 



2. 2 » » » + 0,5 » » » (20 X * ) + » 



3. 2 » » » 4- 0,5 » » » (40 X » ) + » 



4. 2 » » » + 0,5 » » » (80 X >> ) + » 



5. 2 » » » + 0,5 » Na CI. -|- fibrine (to be contrôle!) 

 6, 7, 8, 9 and 10 are to be prepared in the same way from serum B. 



II. 1,5 cM». NaCl. + 0,5 cM'. Serum A (10 X diluted) + 0,5 cM^. 10O/0T+ fibrine. 



1,5 » » » +0,5 » » » (20 X » ) + 0,5 » » + » 



1,5 » » » + 0,5 » » » (40 X » ) + Oi5 » » + » 



1,5 » » » + 0,5 » » » (80 X » ) + o,S » » + » 



1,5 » » » +0,5 » NaCl. + 0,5 cM3. io°/o T + fibrine (contrôle!) 



12 

 13 



'4 

 15 

 16, 17, 18, 19 and 20 are to be prepared in the same way from serum B. 



For the preparation of the different mixtures, the dilutions 

 are first prepared, then the tuberculine-solution is added. Then 

 the mixtures serum-tuberculine are placed in the incubator for 

 15 minutes in a temperature of ßj^'C. Finally the pieces of 

 fibrine are added. For experience teaches that when serum is 

 first allowed to act upon tuberculine, the tuberculosis-serum is 

 still less checked in its fibrolytic power by tuberculine than 

 non-tuberculous serum., 



As we see, all sera are diluted 50, 100, 200 and 400 times. 

 To the second series a 10 °/o tuberculine solution is added, 

 but, as this is 5 times diluted, the T solution is 2 0/0. From 



